The ARHGEF12 Antibody (PAC022572) is a valuable tool for researchers studying the ARHGEF12 protein, which plays a key role in cell signaling pathways and cytoskeletal organization. This polyclonal antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.ARHGEF12, also known as rho guanine nucleotide exchange factor 12, is involved in the regulation of cell migration, cell shape, and cell adhesion processes. Dysregulation of ARHGEF12 has been implicated in diseases such as cancer, cardiovascular disorders, and neurological disorders.
By targeting ARHGEF12, researchers can gain insight into the molecular mechanisms driving these diseases and potentially identify novel therapeutic targets.The ARHGEF12 Antibody enables the detection and analysis of ARHGEF12 protein expression in various cell types, making it a valuable tool for studies in cell biology and disease research. Understanding the role of ARHGEF12 in cellular processes and disease pathogenesis is essential for advancing our knowledge of these complex conditions and developing effective treatments.
Antibody Name:
ARHGEF12 Antibody (PACO22572)
Antibody SKU:
PACO22572
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human ARHGEF12.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from RAW264.7 cells, using ARHGEF12 antibody.
Background:
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
ARHGEF12: a widely-expressed guanine nucleotide exchange factor (GEF) for RhoA GTPase. May act as GTPase-activating protein (GAP) for G-alpha 12 and 13. The fusion protein MLL-ARHGEF12 appears to be a disease driver in acute myeloid leukemia. Two isoforms of the human protein are produced by alternative splicing.Protein type: GEF; GEF, Rac/Rho; Motility/polarity/chemotaxis; OncoproteinChromosomal Location of Human Ortholog: 11q23.3Cellular Component: cytoplasm; cytosolMolecular Function: G-protein-coupled receptor binding; guanyl-nucleotide exchange factor activity; protein binding; Rho guanyl-nucleotide exchange factor activityBiological Process: G-protein coupled receptor protein signaling pathway; positive regulation of apoptosis; regulation of small GTPase mediated signal transduction
UniProt Protein Details:
NCBI Summary:
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
