The ARNT Polyclonal Antibody (CAB0972) is a valuable tool for researchers studying the Aryl hydrocarbon receptor nuclear translocator (ARNT), a protein involved in regulating gene expression in response to environmental stimuli. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting ARNT in human samples, and has been validated for use in Western blot applications.ARNT is a key transcription factor that forms heterodimers with other proteins to regulate the expression of genes involved in various physiological processes, including metabolism, cell growth, and differentiation.
Its role in mediating the cellular response to environmental toxins and carcinogens makes it a crucial target for studies in toxicology, cancer biology, and environmental health.By using the ARNT Polyclonal Antibody, researchers can confidently explore the function and regulation of ARNT in different cell types and experimental conditions, providing valuable insights into its role in health and disease. This antibody is a valuable asset for investigations into the molecular mechanisms underlying ARNT-mediated gene expression and its implications for human health.
Product Name:
HIF1β/ARNT Rabbit Polyclonal Antibody
SKU:
CAB0972
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 420-485 of human HIF1β/ARNT (NP_001659.1).
This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
405
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HIF1β/ARNT antibody (CAB0972) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
