The ARPC3 Antibody (CAB7767) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. RRID AB_2768454 Gene ID 10094 Swiss Prot Synonym ARC21; p21-Arc; ARPC3
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ARPC3 Antibody
SKU:
CAB7767
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARC21, p21-Arc, ARPC3
Positive Sample:
U-251MG, SKOV3, HeLa, SH-SY5Y, Mouse spleen, Mouse lung, Rat ovary
Cellular Localization:
Cell Projection, Cytoplasm, Cytoskeleton.
Calculated MW:
21kDa
Observed MW:
21kDa
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells. Alternatively spliced transcript variants have been found for this gene. RRID AB_2768454 Gene ID 10094 Swiss Prot Synonym ARC21; p21-Arc; ARPC3
Purification Method:
Affinity purification
Gene ID:
10094
RRID:
AB_2768454
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ARPC3 Rabbit pAb (CAB7767) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of A549 cells using ARPC3 Rabbit pAb (CAB7767). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
