The ATF7 Antibody (CAB7580) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for ATF7 and is suitable for use in Western blot applications. By binding to ATF7 protein, this antibody enables the detection and analysis of ATF7 expression in various cell types, making it ideal for investigations in molecular biology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATF7 Antibody
SKU:
CAB7580
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MGDD RPFV CNAP GCGQ RFTN EDHL AVHK HKHE MTLK FGPA RTDS VIIA DQTP TPTR FLKN CEEV GLFN ELAS SFEH EFKK AADE DEKK ARSR TVAK KLVV FRPR LFLL CFGI IFLI G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATFA, ATF7
Positive Sample:
BT-474, SW620, HepG2
Cellular Localization:
Cytoplasm, Nucleus, Nucleoplasm.
Calculated MW:
52kDa
Observed MW:
53kDa
Enables several functions, including DNA-binding transcription repressor activity, RNA polymerase II-specific; mitogen-activated protein kinase binding activity; and transcription coactivator binding activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Biomarker of colorectal cancer.
Purification Method
Affinity purification
Gene ID
11016
RRID
AB_2768102
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ATF7 Rabbit pAb (CAB7580) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
