The ATN1 Antibody (PAC02183) is a valuable tool for researchers studying ATN1, a protein involved in the pathogenesis of multiple neurodegenerative disorders, including dentatorubral-pallidoluysian atrophy (DRPLA) and Huntington's disease. This polyclonal antibody, produced by Assay Genie, has been rigorously tested for its reactivity with human samples in various applications such as Western blotting.ATN1, a transcriptional corepressor, plays a critical role in regulating gene expression and neuronal development.
Dysregulation of ATN1 has been implicated in the pathogenesis of neurodegenerative diseases, making it an important target for research in the field of neuroscience and neurology. By using the ATN1 Antibody, researchers can detect and analyze ATN1 protein levels in different cell types, providing valuable insights into its function and potential therapeutic strategies for treating neurodegenerative disorders.
Antibody Name:
ATN1 Antibody (PACO22183)
Antibody SKU:
PACO22183
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from N-terminal of human ATN1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells, A549 cells, K562 cells, COLO205 cells and NIH-3T3 cells, using ATN1 antibody.
Background:
Transcriptional corepressor. Recruits NR2E1 to repress transcription. Promotes vascular smooth cell (VSMC) migration and orientation By similarity. Corepressor of MTG8 transcriptional repression. Has some intrinsic repression activity which is independent of the number of poly-Asn (polyQ) repeats.
DRPLA: a protein that interacts with the E3 ubiquitin-protein ligase WWP1 and WWP2. May be involved in the third step of ubiquitin conjugation. Relatively high levels in the brain, ovary, testis and prostate. Lower levels in the liver, thymus and leukocytes. Defects are the cause of the neurodegenerative disorders dentatorubral-pallidoluysian atrophy (DRPLA) and Haw River syndrome (HRS).Protein type: Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 12p13.31Cellular Component: cell junction; cytoplasm; nuclear matrix; nucleoplasm; nucleus; perinuclear region of cytoplasmMolecular Function: protein binding; protein domain specific binding; transcription corepressor activityBiological Process: central nervous system development; negative regulation of transcription from RNA polymerase II promoter; neuron apoptosis; transcription, DNA-dependentDisease: Dentatorubral-pallidoluysian Atrophy
UniProt Protein Details:
NCBI Summary:
Dentatorubral pallidoluysian atrophy (DRPLA) is a rare neurodegenerative disorder characterized by cerebellar ataxia, myoclonic epilepsy, choreoathetosis, and dementia. The disorder is related to the expansion from 7-23 copies to 49-75 copies of a trinucleotide repeat (CAG/CAA) within this gene. The encoded protein includes a serine repeat and a region of alternating acidic and basic amino acids, as well as the variable glutamine repeat. Alternative splicing results in two transcripts variants that encode the same protein. [provided by RefSeq, Feb 2010]
