The ATP12A Antibody (CAB14372) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, offers high reactivity with human samples and is validated for use in various applications, including Western blot and immunofluorescence.ATP12A plays a crucial role in maintaining cellular pH balance and regulating ion levels, making it a key player in processes such as endocytosis, phagocytosis, and lysosomal function. Dysregulation of ATP12A has been linked to diseases such as hypertension, gastric cancer, and kidney disorders, highlighting its importance in physiological and pathological conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATP12A Antibody
SKU:
CAB14372
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HK, ATP1AL1, H-K-ATPase, ATP12A
Positive Sample:
A-431, 293T
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
116kDa
Observed MW:
116kDa
The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This gene encodes a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
479
RRID
AB_2761239
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ATP12A Rabbit pAb (CAB14372) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
