The SERCA1/ATP2A1 Antibody (CAB10104) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the ATP2A1 protein, this antibody allows for the detection and analysis of ATP2A1 expression in various cell types, making it ideal for research in cell biology and calcium signaling.ATP2A1, also known as SERCA1, is responsible for maintaining calcium homeostasis in the endoplasmic reticulum of cells, which is essential for various cellular functions including muscle contraction and neurotransmitter release.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
SERCA1/ATP2A1 Antibody
SKU:
CAB10104
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
487
RRID
AB_2757627
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C2C12 cells, using SERCA1/ATP2A1 Rabbit pAb (CAB10104) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
