Description
ATP5G2 Antibody (CAB18279)
The ATP5G2 Antibody (CAB18279) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to ATP5G2, this antibody enables the detection and analysis of the protein in various cell types, making it ideal for investigations in bioenergetics, metabolism, and mitochondrial dysfunction.ATP5G2 is essential for the synthesis of ATP, the primary energy currency of cells, and plays a crucial role in cellular respiration.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | ATP5G2 Antibody |
| SKU: | CAB18279 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||||
| Sequence: | ATAP HPLK MFAC SKFV STPS LVKS TSQL LSRP LSAV VLKR PEIL TDES LSSL AVSC PLTS LVSS RSFQ TSAI SRDI DTAA KFIG AGAA TVGV AGSG AGIG T | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
| ||||||
| Synonyms: | ATP5A, ATP5G2 |
| Positive Sample: | Mouse kidney, Mouse brain |
| Cellular Localization: | Mitochondrial Inner Membrane, Mitochondrial Outer Membrane, Mitochondrial Proton-Transporting Atp Synthase Complex, Mitochondrial Proton-Transporting Atp Synthase Complex, Coupling Factor F(O). |
| Calculated MW: | 15kDa |
| Observed MW: | 16kDa |
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). There are three separate genes which encode subunit c of the proton channel and they specify precursors with different import sequences but identical mature proteins. The protein encoded by this gene is one of three precursors of subunit c. This gene has multiple pseudogenes.
| Purification Method | Affinity purification |
| Gene ID | 517 |
| RRID | AB_2862053 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using ATP5G2 Rabbit pAb (CAB18279) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using ATP5G2 Rabbit pAb (CAB18279) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
