The ATP6 Monoclonal Antibody (CAB23150) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, generated using hybridoma technology, demonstrates exceptional reactivity to human ATP6 and is validated for use in various applications, including Western blotting and immunofluorescence.ATP6, also known as the subunit a of V-ATPase, plays a crucial role in maintaining cellular pH balance and promoting lysosomal acidification. Dysregulation of ATP6 function has been linked to various pathological conditions, including neurodegenerative diseases and cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATP6 Monoclonal Antibody
SKU:
CAB23150
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC59849
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted to contribute to proton-transporting ATP synthase activity, rotational mechanism. Predicted to be involved in mitochondrial ATP synthesis coupled proton transport. Located in mitochondrion. Is expressed in several structures, including alimentary system; brain; heart; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in Leber hereditary optic neuropathy; NARP syndrome; Parkinson's disease; multiple sclerosis; and systemic lupus erythematosus. Orthologous to human MT-ATP6 (mitochondrially encoded ATP synthase membrane subunit 6).
Purification Method
Affinity purification
Gene ID
17705
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ATP6 Rabbit mAb (CAB23150) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
