Description
ATP6V1A Antibody (CAB14707)
The ATP6V1A Antibody (CAB14707) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.By binding to the ATP6V1A protein, this antibody enables the detection and analysis of ATP6V1A in various cell types, making it an essential tool for studies in cell biology and molecular biology. The ATP6V1A protein is involved in multiple cellular processes, including endocytosis, protein degradation, and autophagy, highlighting its importance in maintaining cellular homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
| Product Name: | ATP6V1A Antibody |
| SKU: | CAB14707 |
| Size: | 20μL, 100μL |
| Reactivity: | Mouse |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | SLAE TDKI TLEV AKLI KDDF LQQN GYTP YDRF CPFY KTVG MLSN MIAF YDMA RRAV ETTA QSDN KITW SIIR EHMG DILY KLSS MKFK DPLK DGEA KIKS D | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | HO68, VA68, VPP2, Vma1, DEE93, ARCL2D, ATP6A1, IECEE3, ATP6V1A1, ATP6V1A |
| Positive Sample: | Mouse brain |
| Cellular Localization: | Apical Plasma Membrane, Cytosol, Extracellular Exosome, Microvillus, Nucleoplasm, Plasma Membrane. |
| Calculated MW: | 68kDa |
| Observed MW: | 74kDa |
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
| Purification Method | Affinity purification |
| Gene ID | 523 |
| RRID | AB_2761582 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from mouse brain, using ATP6V1A Rabbit pAb (CAB14707) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min. |
