Description
ATP6V1D Antibody (CAB12940)
The ATP6V1D Antibody (CAB12940) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with samples from various species and has been validated for use in Western blot and immunohistochemistry applications.ATP6V1D plays a crucial role in maintaining cellular pH balance and regulating membrane transport processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | ATP6V1D Antibody |
| SKU: | CAB12940 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | MSGK DRIE IFPS RMAQ TIMK ARLK GAQT GRNL LKKK SDAL TLRF RQIL KKII ETKM LMGE VMRE AAFS LAEA KFTA GDFS TTVI QNVN KAQV KIRA KKDN VAGV TLPV FEHY HEGT DSYE LTGL ARGG EQLA KLKR NYAK AVEL LVEL ASLQ TSFV TLDE AIKI TNRR VNAI EHVI IPRI ERTL AYII TELD ERER EEFY RLKK IQEK KKIL KEKS EKDL EQRR AAGE VLEP ANLL AEEK DEDL LFE | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
| ||||
| Synonyms: | VATD, VMA8, ATP6M, ATP6V1D |
| Positive Sample: | U-87MG, 293T, HeLa, Mouse lung, Mouse brain |
| Cellular Localization: | Cytoplasmic Side, Membrane, Peripheral Membrane Protein. |
| Calculated MW: | 28kDa |
| Observed MW: | 35kDa |
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein.
| Purification Method | Affinity purification |
| Gene ID | 51382 |
| RRID | AB_2759787 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using ATP6V1D Rabbit pAb (CAB12940) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s. |
