The ATP6V1F Polyclonal Antibody (PAC028546) is a valuable tool for researchers studying ATP6V1F, a subunit of the V-ATPase complex involved in various cellular processes. This antibody is raised in rabbits and is specifically targeted towards human samples, making it ideal for experiments focusing on ATP6V1F in human cells.The ATP6V1F antibody has been validated for use in Western blot applications, allowing for the detection and analysis of ATP6V1F protein levels in different cell types. Researchers in the fields of molecular biology, cell biology, and biochemistry will find this antibody particularly useful for studying the function and regulation of ATP6V1F in cellular processes such as vesicular trafficking, protein sorting, and organelle acidification.
Given the crucial role of ATP6V1F in cellular physiology, this antibody can provide key insights into the mechanisms underlying various diseases, including cancer, neurodegenerative disorders, and metabolic diseases. By understanding the functions of ATP6V1F, researchers can potentially develop targeted therapies that modulate its activity for therapeutic purposes.
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO28546 at dilution of 1:100.
Immunofluorescent analysis of MCF-7 cells using PACO28546 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acid, fying a variety of intracellular compartments in eukaryotic cells.
ATP6V1F: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase F subunit family.Protein type: HydrolaseChromosomal Location of Human Ortholog: 7q32Cellular Component: cytosol; membrane; vacuolar proton-transporting V-type ATPase complexMolecular Function: ATPase activity, coupled to transmembrane movement of ions; protein bindingBiological Process: insulin receptor signaling pathway; transferrin transport
UniProt Protein Details:
NCBI Summary:
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq, Jul 2008]
