The ATR Antibody (CAB7247) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting ATR in human samples, making it an invaluable tool for experiments involving Western blotting.ATR is a crucial protein involved in maintaining genomic stability and preventing the accumulation of mutations that can lead to cancer development. By targeting ATR with this polyclonal antibody, researchers can gain insights into the role of ATR in DNA damage repair and cell cycle checkpoint control.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ATR Antibody
SKU:
CAB7247
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FRP1, MEC1, SCKL, FCTCS, SCKL1, ATR
Positive Sample:
NCI-H460
Cellular Localization:
Chromosome, Nucleus, Pml Body.
Calculated MW:
301kDa
Observed MW:
301kDa
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
Purification Method
Affinity purification
Gene ID
545
RRID
AB_2767794
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NCI-H460 cells, using ATR Rabbit pAb (CAB7247) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
