The AUP1 Polyclonal Antibody (PAC015434) is a valuable tool for researchers studying the protein AUP1, which is involved in lipid metabolism and ER-associated degradation (ERAD) pathways. This antibody, raised in rabbits, shows high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to AUP1, this antibody allows for the detection and analysis of AUP1 expression in various cell types, making it an excellent choice for research in lipid biology and ER stress pathways.
AUP1 is a key player in maintaining lipid homeostasis and regulating ER stress responses. Dysregulation of AUP1 has been implicated in lipid metabolism disorders, such as non-alcoholic fatty liver disease (NAFLD) and obesity. Additionally, AUP1 has been linked to ER stress-related diseases, including neurodegenerative disorders and diabetes. Understanding the function of AUP1 is essential for unraveling its role in these diseases and developing potential therapeutic interventions.
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO15434(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hela cells, 293T cells, Primary antibody: PACO15434(AUP1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO15434(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.
Synonyms:
ancient ubiquitous protein 1
UniProt Protein Function:
AUP1: May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Belongs to the AUP1 family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Transferase; Membrane protein, integral; Endoplasmic reticulumChromosomal Location of Human Ortholog: 2p13Cellular Component: integral to endoplasmic reticulum membrane; membraneMolecular Function: protein binding; ubiquitin bindingBiological Process: ER-associated protein catabolic process; retrograde protein transport, ER to cytosol
UniProt Protein Details:
NCBI Summary:
The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
