The AUP1 Polyclonal Antibody (PAC015435) is a vital tool for researchers studying AUP1, a protein involved in lipid metabolism and ER-associated degradation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By binding to the AUP1 protein, this antibody enables accurate detection and analysis in various cell types, making it perfect for investigations in cell biology and metabolic research.
AUP1 is a key player in maintaining cellular lipid homeostasis and protein quality control within the endoplasmic reticulum. Its function in lipid droplet formation and ER stress response makes it a promising target for studying metabolic disorders, such as obesity and lipid storage diseases. Understanding the role of AUP1 is essential for developing potential therapies that target lipid metabolism and ER stress-related pathways.
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO15435(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Hela cells, 293T cells, mouse liver tissue, NIH/3T3 cells, Primary antibody: PACO15435(AUP1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO15435(AUP1 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.
Synonyms:
ancient ubiquitous protein 1
UniProt Protein Function:
AUP1: May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Belongs to the AUP1 family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Transferase; Membrane protein, integral; Endoplasmic reticulumChromosomal Location of Human Ortholog: 2p13Cellular Component: integral to endoplasmic reticulum membrane; membraneMolecular Function: protein binding; ubiquitin bindingBiological Process: ER-associated protein catabolic process; retrograde protein transport, ER to cytosol
UniProt Protein Details:
NCBI Summary:
The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
