The AVIL Antibody (CAB15785) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human cell samples and has been validated for use in Western blot applications. By binding specifically to the Avil protein, researchers can accurately detect and analyze Avil expression in a variety of cell types, making it ideal for studies in cell biology and cancer research.Avil is known for its role in regulating actin filament assembly, cell migration, and cell adhesion, making it a critical player in processes such as wound healing and cancer metastasis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AVIL Antibody
SKU:
CAB15785
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p92, DOC6, ADVIL, NPHS21, AVIL
Positive Sample:
HT-29, Mouse uterus, Mouse brain, Rat uterus, Rat testis, Rat brain
Cellular Localization:
Cell Projection, Cytoplasm, Axon, Cytoskeleton.
Calculated MW:
92kDa
Observed MW:
105kDa,115kDa
The protein encoded by this gene is a member of the gelsolin/villin family of actin regulatory proteins. This protein has structural similarity to villin. It binds actin and may play a role in the development of neuronal cells that form ganglia.
Purification Method
Affinity purification
Gene ID
10677
RRID
AB_2763205
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using AVIL Rabbit pAb (CAB15785) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of L929 cells using AVIL Rabbit pAb (CAB15785) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
