Description
AXIN2 Antibody (CAB17021)
The AXIN2 Antibody (CAB17021) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific and reacts with human samples, making it ideal for applications such as Western blotting and immunofluorescence.Axin2 is a crucial component of the Wnt signaling pathway, which plays a critical role in embryonic development, tissue homeostasis, and cancer progression. Dysregulation of Axin2 expression has been implicated in a variety of diseases, including colorectal cancer, breast cancer, and osteoporosis.By utilizing the Axin2 Polyclonal Antibody, researchers can investigate the expression levels and localization of Axin2 in various cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
| Product Name: | AXIN2 Antibody |
| SKU: | CAB17021 |
| Size: | 20μL, 100μL |
| Reactivity: | Mouse, Rat |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | EDEE REGS ELTL NSRE GAPT QHPL SLLP SGSY EEDP QTIL DDHL SRVL KTPG CQSP GVGR YSPR SRSP DHHH HHHS QYHS LLPP GGKL PPAA ASPG ACPL L | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | AXIL, ODCRCS, AXIN2 |
| Positive Sample: | Mouse brain, Rat brain |
| Cellular Localization: | Cytoplasm. |
| Calculated MW: | 94kDa |
| Observed MW: | 110kDa |
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
| Purification Method | Affinity purification |
| Gene ID | 8313 |
| RRID | AB_2768531 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using AXIN2 Rabbit pAb (CAB17021) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
