The Axl Monoclonal Antibody (CAB22378) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using a hybridoma fusion technique, is highly specific for detecting AXL protein in human samples and is validated for use in immunohistochemistry, immunofluorescence, and flow cytometry applications.AXL is known to be overexpressed in many cancers and has been implicated in promoting tumor growth, metastasis, and drug resistance. By targeting AXL with this antibody, researchers can gain valuable insights into the role of AXL in cancer biology and potentially identify novel therapeutic strategies for treating AXL-driven malignancies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Axl Monoclonal Antibody
SKU:
CAB22378
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC53006
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARK, UFO, JTK11, Tyro7, Axl
Positive Sample:
HeLa, Mouse heart
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
98kDa
Observed MW:
138kDa
The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. The encoded protein acts as a host cell receptor for multiple viruses, including Marburg, Ebola and Lassa viruses and is a candidate receptor for the SARS-CoV2 virus.
Purification Method
Affinity purification
Gene ID
558
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Axl Rabbit mAb (CAB22378) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Mouse heart, using Axl Rabbit mAb (CAB22378) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
