The Bad Polyclonal Antibody (CAB24658) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the BAD protein, this antibody allows for the detection and analysis of BAD in various cell types, making it ideal for research in apoptosis, cancer, and other related fields.BAD, also known as Bcl-2-associated death promoter, plays a crucial role in the regulation of programmed cell death by promoting apoptosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Bad Polyclonal Antibody
SKU:
CAB24658
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BBC2, BCL2L8, ad
Positive Sample:
HeLa, 293T, Mouse brain, Mouse ovary
Cellular Localization:
Cytoplasm, Mitochondrion Outer Membrane.
Calculated MW:
18kDa
Observed MW:
23kDa
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Purification Method
Affinity purification
Gene ID
572
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Bad Rabbit pAb (CAB24658) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from wild type (WT) and Bad knockdown (KD) 293T(KD) cells, using Bad Rabbit pAb (CAB24658) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
