The BBOX1 Antibody (CAB5810) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the BBOX1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in metabolism and energy production.BBOX1 is essential for the synthesis of carnitine, a molecule critical for the transport of fatty acids into the mitochondria for energy production. Dysregulation of BBOX1 has been linked to various metabolic disorders, making it a potential target for therapeutic interventions.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BBOX1 Antibody
SKU:
CAB5810
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BBH, BBOX, G-BBH, gamma-BBH, BBOX1
Positive Sample:
Mouse testis, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
45kDa
Observed MW:
45kDa
This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation.
Purification Method
Affinity purification
Gene ID
8424
RRID
AB_2766562
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BBOX1 Rabbit pAb (CAB5810) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse kidney using BBOX1 Rabbit pAb (CAB5810) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
