The BCKDK Monoclonal Antibody (CAB20909) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has high reactivity with human samples, making it suitable for use in Western blot applications. By binding to the BCKDK protein, this antibody enables the detection and analysis of BCKDK in various cell types, offering researchers valuable insights into metabolic processes and potential therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BCKDK Monoclonal Antibody
SKU:
CAB20909
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2875
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MILA SVLR SGPG GGLP LRPL LGPA LALR ARST SATD THHV EMAR ERSK TVTS FYNQ SAID AAAE KPSV RLTP TMML YAGR SQDG SHLL KSAR YLQQ ELPV
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BDK, BCKDKD, BCKDK
Positive Sample:
HeLa, HepG2, PC-12, Mouse pancreas
Cellular Localization:
Mitochondrion, Mitochondrion Matrix.
Calculated MW:
46kDa
Observed MW:
46kDa
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10295
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using BCKDK Rabbit mAb (CAB20909) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
