The Bcl-2 Monoclonal Antibody (CAB19693) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting BCL-2 in human samples. It has been validated for use in Western blot applications, enabling precise analysis of BCL-2 expression levels in various cellular contexts.BCL-2 is a pivotal player in the apoptotic pathway, promoting cell survival by preventing the activation of pro-apoptotic factors. Dysregulation of BCL-2 has been implicated in a variety of diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Bcl-2 Monoclonal Antibody
SKU:
CAB19693
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0173
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
596
RRID
AB_2862738
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Bcl-2 Rabbit mAb (CAB19693) at 1:7000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
Western blot analysis of lysates from wild type (WT) and Bcl-2 knockdown (KD) 293T cells using Bcl-2 Rabbit mAb (CAB19693) at 1:7000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Bcl-2 Rabbit mAb (CAB19693) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunoprecipitation analysis of 200 μg extracts of Mouse lung using 3 μg Bcl-2 Rabbit mAb (CAB19693). Western blot was performed from the immunoprecipitate using Bcl-2 Rabbit mAb (CAB19693) at a dilution of 1:1000.
