Description
BCL2 Recombinant Monoclonal Antibody (RACO0592)
The BCL2 Recombinant Monoclonal Antibody (RACO0592) is a powerful tool for researchers studying BCL2, a key regulator of apoptosis and cell survival. This antibody, developed using recombinant technology, offers high specificity and sensitivity for detecting BCL2 protein in various cell types. It is validated for use in applications such as Western blotting, immunofluorescence, and immunohistochemistry.BCL2 is a critical protein in the regulation of programmed cell death, with important implications in cancer and other diseases.
By targeting BCL2, researchers can gain insights into the mechanisms of cell survival and death, as well as potential therapeutic strategies for cancer treatment. The BCL2 Recombinant Monoclonal Antibody provides a reliable tool for studying BCL2 expression and function, making it an essential component in cancer research and drug development efforts.
| Product Name: | BCL2 Recombinant Monoclonal Antibody | ||||||
| SKU: | RACO0592 | ||||||
| Size: | 50uL, 100uL | ||||||
| UniProt No: | P10415 | ||||||
| Target Names: | BCL2 | ||||||
| Alternative Names: | BCL2; Apoptosis regulator Bcl-2 | ||||||
| Species Reactivity: | Human, Mouse, Rat | ||||||
| Immunogen: | A synthesized peptide | ||||||
| Immunogen Species: | Homo sapiens (Human) | ||||||
| Clonality: | Monoclonal | ||||||
| Purification Method: | Affinity-chromatography | ||||||
| Conjugate: | Non-conjugated | ||||||
| Form: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | ||||||
| Storage: | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||
| Lead Time: | Basically, we can dispatch the products out in 7-10 working days after receiving your orders. Delivery time may differ depending on the purchasing method or location. Please consult your local distributors for specific delivery times. | ||||||
| Tested Applications: | ELISA WB IHC FC | ||||||
| Recommended Dilution: |
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| Description: | The production of the BCL2 recombinant monoclonal antibody involves the utilization of protein technology and DNA recombinant techniques. In this process, animals are initially immunized with a synthesized peptide derived from human BCL2, and B cells are extracted from the immunized mice. From these B cells, positive ones are isolated and subjected to single clone identification. The genes responsible for the light and heavy chains of the BCL2 antibody are then amplified using PCR and incorporated into a plasmid vector. This recombinant vector is subsequently introduced into host cells for antibody expression. Through affinity chromatography, the BCL2 recombinant monoclonal antibody is purified from the supernatant of the cell culture. Rigorous validation ensures the reliability and applicability of the antibody in various experimental techniques, including ELISA, WB, IHC, and FC. This antibody is designed to specifically target the Bcl-2 protein in human, mouse, and rat species. |
| Function: | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. |
| Gene References into Functions: |
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| Subcellular Location: | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
| Protein Families: | Bcl-2 family |
| Tissue Specificity: | Expressed in a variety of tissues. |
| Database Links: | HGNC: 990 OMIM: 151430 KEGG: hsa:596 STRING: 9606.ENSP00000329623 UniGene: Hs.150749 |
 | Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate All lanes: BCL2 antibody at 1μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa |
 | IHC image diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Overlay histogram showing Jurkat cells stained with CSB-RA002611A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
