The BHMT Rabbit Monoclonal Antibody (CAB5134) is a cutting-edge tool for researchers studying the role of BHMT, a key enzyme involved in homocysteine metabolism and one-carbon metabolism pathways. This highly specific antibody, produced in rabbits, is optimized for use in various research applications, particularly in Western blot analyses. By binding to the BHMT protein, this antibody enables precise detection and analysis in a wide range of sample types, making it an essential tool for studies in biochemistry, metabolic disorders, and liver function.
The BHMT enzyme plays a crucial role in maintaining homocysteine levels and methyl group donation, making it a significant player in metabolic processes and epigenetic regulation. Dysregulation of BHMT activity has been linked to various health conditions, including cardiovascular disease, neural tube defects, and liver dysfunction. By studying BHMT expression and function with the help of this antibody, researchers can gain valuable insights into these disorders and potentially identify new therapeutic targets for intervention.
Product Name:
BHMT Rabbit Monoclonal Antibody
SKU:
CAB5134
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-406 of human BHMT (Q93088).
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Purification Method:
Affinity purification
Gene ID:
635
Clone Number:
ARC1249
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using BHMT Rabbit mAb (CAB5134) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180S.
