The Bim Monoclonal Antibody (CAB19702) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting Bim in various experimental setups.Recognized for its ability to bind specifically to Bim, the antibody is optimized for use in Western blot applications, enabling accurate analysis of Bim expression levels in different cell types and tissues. Its reliability and consistency make it an essential component for studies focused on understanding the mechanisms underlying cell death and survival pathways.Bim, a member of the Bcl-2 protein family, is involved in controlling cell death by promoting apoptosis in response to various cellular signals.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Bim Monoclonal Antibody
SKU:
CAB19702
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0182
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.
Purification Method
Affinity purification
Gene ID
10018
RRID
AB_2862744
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Bim Rabbit mAb (CAB19702) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Western blot analysis of various lysates using Bim Rabbit mAb (CAB19702) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
