Biotinylated Recombinant Cynomolgus Mesothelin/MSLN Protein (Primary Amine Labeling)
The Biotinylated Recombinant Cynomolgus Mesothelin/MSLN Protein (Primary Amine Labeling) is a specially modified recombinant protein featuring biotin conjugation for enhanced detection and purification capabilities. This biotinylated variant is particularly valuable in immunoassay development, protein interaction studies, flow cytometry applications, and biosensor technologies, offering researchers improved sensitivity and specificity in experimental workflows requiring streptavidin-biotin binding systems.
This product (SKU: RPCB0088) is produced using advanced expression systems and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 33 kD with an observed molecular weight of 35-50 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Cynomolgus Mesothelin/MSLN Protein (Primary Amine Labeling)
SKU:
RPCB0088
Size:
100 μg
Reactivity:
Cynomolgus
Synonyms:
MSLN, CAK1, Mesothelin, MPF, MPFSMRP, SMR
Tag:
C-His
Calculated MW:
33 kD
Observed MW:
35-50 kDa
Protein Description:
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Cynomolgus Mesothelin/MSLN Protein (Primary Amine Labeling) (RPCB0088), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Biotinylated Recombinant Cynomolgus Mesothelin/MSLN Protein (Primary Amine Labeling) was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Cynomolgus MSLN is greater than 95% as determined by SEC-HPLC.
