Biotinylated Recombinant Human ALCAM/CD166 Protein (Primary Amine Labeling)
The Biotinylated Recombinant Human ALCAM/CD166 Protein (Primary Amine Labeling) is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated ALCAM/CD166 (Primary Amine Labeling) function and its implications in health and disease.
This product (SKU: RPCB0668) is produced using advanced expression systems and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 57 kDa with an observed molecular weight of 70-80 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human ALCAM/CD166 Protein (Primary Amine Labeling)
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human ALCAM/CD166 Protein (Primary Amine Labeling) (RPCB0668), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM.
Biotinylated Recombinant Human ALCAM/CD166 Protein (Primary Amine Labeling) was determined by Tris-Bis PAGE under reducing conditions.
Immobilized Anti-ALCAM Antibody, hFc Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human ALCAM, His Tag with the EC50 of 1.6 μg/mL determined by ELISA.
