Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2 (275-496) Protein
The Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2 (275-496) Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated Angiopoietin-2/ANG-2/ANGPT2 (275-496) function and its implications in health and disease.
This product (SKU: RPCB0047) is produced using advanced expression systems and features a N-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 28.4 kDa with an observed molecular weight of 32-37 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2 (275-496) Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2 (275-496) Protein (RPCB0047), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length. Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
Biotinylated Recombinant Human Angiopoietin-2/ANG-2/ANGPT2 (275-496) Protein was determined by Tris-Bis PAGE under reducing conditions.
Immobilized Biotinylated Human ANGPT2, His Tag at 5μg/ml (100μl/Well) on the Streptavidin Procated plate. Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
