Biotinylated Recombinant Human CSF1R/M-CSF R/CD115 Protein
The Biotinylated Recombinant Human CSF1R/M-CSF R/CD115 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated CSF1R/M-CSF R/CD115 function and its implications in health and disease.
This product (SKU: RPCB0100) is produced using advanced expression systems and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 57.4 kDa with an observed molecular weight of 75-105 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human CSF1R/M-CSF R/CD115 Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human CSF1R/M-CSF R/CD115 Protein (RPCB0100), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Immobilized Anti-CSF R Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human M-CSF R, His Tag with the EC50 of 45.4ng/ml determined by ELISA.
Biotinylated Human M-CSF R, His-Avi Tag immobilized on CM5 Chip can bind Human M-CSF, No Tag with an affinity constant of 0.81 μM as determined in SPR assay (Biacore T200).
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
Biotinylated Recombinant Human CSF1R/M-CSF R/CD115 Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human M-CSF R is greater than 95% as determined by SEC-HPLC.
Biotinylated Human M-CSF R, His-Avi Tag immobilized on CM5 Chip can bind Human M-CSF, No Tag with an affinity constant of 0.81 μM as determined in SPR assay (Biacore T200).
