The Biotinylated Recombinant Human CTGF/CCN2 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated CTGF/CCN2 function and its implications in health and disease.
This product (SKU: RPCB0072) is produced using advanced expression systems and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 38.4 kDa with an observed molecular weight of 43-53 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE., ensuring exceptional quality and consistency for research applications.
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human CTGF/CCN2 Protein (RPCB0072), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, 200 mM L-arginine, pH 7.4.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis.CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.
Biotinylated Recombinant Human CTGF/CCN2 Protein was determined by Tris-Bis PAGE under reducing conditions.
Immobilized Anti-CTGF Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CTGF, His Tag with the EC50 of 32.6ng/ml determined by ELISA.
