Biotinylated Recombinant Human Low-density lipoprotein receptor/LDLR Protein
The Biotinylated Recombinant Human Low-density lipoprotein receptor/LDLR Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated Low-density lipoprotein receptor/LDLR function and its implications in health and disease.
This product (SKU: RPCB0117) is produced using advanced expression systems and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 87.6 kDa with an observed molecular weight of 120-140 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human Low-density lipoprotein receptor/LDLR Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human Low-density lipoprotein receptor/LDLR Protein (RPCB0117), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃. Store the lyophilized protein at -20℃ to -80℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.
Biotinylated Recombinant Human Low-density lipoprotein receptor/LDLR Protein was determined by Tris-Bis PAGE under reducing conditions with Coomassie Blue.
The purity of Biotinylated Human LDLR is greater than 95% as determined by SEC-HPLC.
