Biotinylated Recombinant Human TNFRSF5/CD40 Protein
The Biotinylated Recombinant Human TNFRSF5/CD40 Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating Biotinylated TNFRSF5/CD40 function and its implications in health and disease.
This product (SKU: RPCB0646) is produced using advanced expression systems and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 22.1 kDa with an observed molecular weight of 35-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC., ensuring exceptional quality and consistency for research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant Human TNFRSF5/CD40 Protein
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant Human TNFRSF5/CD40 Protein (RPCB0646), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Biotinylated Recombinant Human TNFRSF5/CD40 Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated Human CD40 is greater than 95% as determined by SEC-HPLC.
Immobilized Anti-CD40 Antibody at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human CD40, His Tag with the EC50 of 31.3ng/mL determined by ELISA.
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag: Human CD40 Ligand (Trimer), hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/mL.
