Biotinylated Recombinant SARS-COV-2 Spike RBD Protein
The Biotinylated Recombinant SARS-COV-2 Spike RBD Protein is a specially modified recombinant protein featuring biotin conjugation for enhanced detection and purification capabilities. This biotinylated variant is particularly valuable in immunoassay development, protein interaction studies, flow cytometry applications, and biosensor technologies, offering researchers improved sensitivity and specificity in experimental workflows requiring streptavidin-biotin binding systems.
This product (SKU: RPCB1350) is produced using HEK293 cells and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 27 kDa with an observed molecular weight of 36-40 kDa under denaturing conditions, achieving ≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography
Mammalian & Bacterial Expression Systems
High lot-to-lot consistency via strict QC
Product Name:
Biotinylated Recombinant SARS-COV-2 Spike RBD Protein
SKU:
RPCB1350
Size:
100 μg
Reactivity:
SARS-COV-2
Synonyms:
S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
High quality, high purity and low endotoxin recombinant Biotinylated Recombinant SARS-COV-2 Spike RBD Protein (RPCB1350), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by Tris-Bis PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Immobilized Human ACE2,His Tag at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Biotinylated SARS COV-2 Spike RBD,His Tag with the EC 50 of 0.097 μg/mL determined by ELISA.
Reconstitution:
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Biotinylated Recombinant SARS-COV-2 Spike RBD Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Biotinylated SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.
