The BLID Antibody (CAB7275) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting BLID in human samples, making it suitable for Western blot applications. By targeting the BLID protein, this antibody allows for the precise identification and analysis of BLID expression in various cell types, offering valuable insights for studies in cancer biology and apoptosis research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BLID Antibody
SKU:
CAB7275
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BRCC2, BRCC2/BLID
Positive Sample:
293T transfected with BRCC2/BLID
Cellular Localization:
Cytoplasm, Mitochondrion.
Calculated MW:
12kDa
Observed MW:
16kDa
This gene encodes a BH3-like motif containing protein involved in cell death. The encoded protein may induce apoptosis in a caspase-dependent manner. The protein is localized in both the cytoplasm and the mitochondrion.
Purification Method
Affinity purification
Gene ID
414899
RRID
AB_2767816
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BRCC2/BLID, using BRCC2/BLID Rabbit pAb (CAB7275) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of MCF-7 cells using BRCC2/BLID Rabbit pAb (CAB7275). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
