The BOK Monoclonal Antibody (CAB21196) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced through advanced cloning techniques, shows high specificity and sensitivity for detecting BOK protein in a variety of experimental setups, including Western blot, immunofluorescence, and immunoprecipitation.BOK, a member of the BCL-2 protein family, is known for its pro-apoptotic functions and its role in maintaining cellular homeostasis by eliminating damaged or unwanted cells. Dysregulation of BOK has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BOK Monoclonal Antibody
SKU:
CAB21196
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC53171
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family.
Purification Method
Affinity purification
Gene ID
666
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from PC-3 cells, using BOK Rabbit mAb (CAB21196) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of various lysates using BOK Rabbit mAb (CAB21196) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
