The Bovine Haptoglobin (HPT) ELISA Kit is a powerful tool for studying and measuring haptoglobin levels in bovine serum, plasma, and tissue samples. This kit offers exceptional sensitivity and specificity, providing accurate and consistent results for various research applications.Haptoglobin is an acute phase protein that plays a crucial role in the immune response and inflammation. Elevated levels of haptoglobin are often associated with conditions such as infection, inflammation, and tissue damage, making it a valuable biomarker for monitoring and diagnosing various health conditions in cattle.
With this ELISA kit, researchers can easily quantify haptoglobin levels in bovine samples, allowing for the investigation of haptoglobin's role in disease progression, immune response, and overall health in cattle. The user-friendly design and reliable performance of this kit make it an essential tool for studying haptoglobin biology and its implications in veterinary research.
Product Name:
Bovine Haptoglobin (Hpt) ELISA Kit
Product Code:
BODL00067
Size:
96 Assays
Target:
Hpt
Synonyms:
HP, Hp2-Alpha, Alpha-2-Macroglobulin, Zonulin
Detection Method:
Sandwich
Range:
156.25-10,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
