Bovine Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit
The Bovine Heat Shock 27kDa Protein (HSPB1) ELISA Kit is specifically designed for the precise measurement of HSPB1 levels in bovine serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.HSPB1, also known as heat shock protein 27 (HSP27), plays a crucial role in cellular stress response and is involved in various physiological processes, including cell survival, apoptosis, and cytoskeleton reorganization.
Dysregulation of HSPB1 has been linked to various diseases, making it a valuable biomarker for studying conditions such as cancer, neurodegenerative disorders, and cardiovascular diseases.By using the Bovine Heat Shock 27kDa Protein (HSPB1) ELISA Kit, researchers can gain valuable insights into the regulation and function of HSPB1 in bovine biology, paving the way for potential therapeutic interventions and disease management strategies.
Product Name:
Bovine Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit
Product Code:
BODL00073
Size:
96 Assays
Target:
HSPB1
Synonyms:
HSP27, HSP-B1, CMT2F, HSP28, Hsp25, Heat Shock Protein 27, 28 kDa heat shock protein, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
