Bovine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
The Bovine Macrophage Inflammatory Protein 3-alpha (MIP-3α) ELISA Kit is a powerful tool for the precise measurement of MIP-3α levels in bovine samples such as serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results that are essential for various research applications.MIP-3α is a key chemokine involved in the recruitment and activation of immune cells, particularly macrophages, in response to inflammation and infection. It plays a crucial role in the immune response and can be implicated in various inflammatory conditions and diseases.
Therefore, the accurate quantification of MIP-3α levels is vital for studying immune system functions, inflammatory processes, and developing potential therapeutic interventions.With its high-quality components and user-friendly protocol, the Bovine MIP-3α ELISA Kit is a valuable tool for researchers looking to investigate the role of MIP-3α in bovine health and disease. Its reliable performance and robust results make it an indispensable asset for any laboratory conducting research in immunology, inflammation, and related fields.
Product Name:
Bovine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Product Code:
BODL00118
Size:
96 Assays
Target:
MIP3a
Synonyms:
CCL20, CKb4, LARC, MIP3-A, SCYA20, ST38, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20, Beta-Chemokine Exodus-1, Liver And Activation-Regulated Chemokine
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
