The Bovine/Sheep Cortisol ELISA Kit is expertly designed for the quantitative detection of cortisol levels in bovine and sheep biological samples.
Cortisol is a vital hormone, produced by the adrenal glands of these animals, which significantly influences the management of stress responses, metabolic processes, and anti-inflammatory actions. It is also involved in various biological activities such as glucose regulation and the metabolism of proteins, fats, and carbohydrates. Precise measurement of cortisol is vital for understanding its role in stress-related disorders, endocrine diseases, or other metabolic complications in bovine and sheep. Assay Genie's Bovine/Sheep Cortisol ELISA Kit delivers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control standards, this kit guarantees robust performance and is straightforward to use, making it an excellent choice for both veterinary research and clinical applications. Trust Assay Genie's Bovine/Sheep Cortisol ELISA Kit for the accurate and dependable quantification of this critical biomarker in your studies.
Product Name:
Bovine/Sheep Cortisol ELISA Kit
SKU:
AEES00017
Target:
Bovine/Sheep Cortisol
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.96 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cortisol. During the reaction, Cortisol in the sample or standard competes with a fixed amount of Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
80-91
88-101
85-103
Average (%)
85
94
94
1:4
Range (%)
82-98
87-96
80-110
Average (%)
87
90
92
1:8
Range (%)
84-101
82-88
86-95
Average (%)
93
85
89
1:16
Range (%)
87-107
89-110
84-95
Average (%)
97
97
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
81-90
85
EDTA plasma (n=5)
82-98
88
Cell culture media (n=5)
85-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.54
100.33
208.22
41.23
102.25
205.71
Standard deviation
3.63
7.68
14.64
3.57
8.17
12.45
C V (%)
8.96
7.65
7.03
8.66
7.99
6.05
Sample type &Sample volume:
serum and plasma; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum and plasma.
Specificity:
This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
