Bovine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit
The Bovine Tissue Inhibitors of Metalloproteinase-2 (TIMP2) ELISA Kit is a highly accurate and sensitive assay designed for the quantitative detection of TIMP2 levels in bovine tissue samples. This kit offers reliable and reproducible results, making it ideal for a variety of research applications.TIMP2 is an important protein involved in the regulation of matrix metalloproteinases (MMPs), key enzymes in extracellular matrix remodeling. Dysregulation of TIMP2 has been linked to various pathological conditions, including cancer, cardiovascular diseases, and inflammatory disorders.
Therefore, accurate measurement of TIMP2 levels is crucial for understanding these diseases and developing potential therapeutic interventions.With its high sensitivity and specificity, the Bovine TIMP2 ELISA Kit provides researchers with a valuable tool for studying the role of TIMP2 in health and disease. Get your kit today and unlock new insights into the intricate mechanisms underlying these conditions.
Product Name:
Bovine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit
Product Code:
BODL00171
Size:
96 Assays
Target:
TIMP2
Synonyms:
CSC-21K, TIMP Metallopeptidase Inhibitor 2
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
