Bovine Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) ELISA Kit
The Bovine Tissue Inhibitors of Metalloproteinase-4 (TIMP4) ELISA Kit from Assay Genie is a cutting-edge tool designed for the accurate measurement of TIMP4 levels in bovine samples. This kit offers unparalleled sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.TIMP4 is a key regulator of metalloproteinases, which play essential roles in tissue remodeling, inflammation, and various disease processes. By accurately measuring TIMP4 levels, researchers can gain valuable insights into the mechanisms underlying these physiological and pathological processes.
This ELISA kit is a valuable asset for studying conditions such as cancer, cardiovascular diseases, and inflammatory disorders, providing a crucial tool for biomarker discovery and therapeutic development.Invest in the Assay Genie Bovine TIMP4 ELISA Kit for robust and reliable results in your research endeavors.
Product Name:
Bovine Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) ELISA Kit
Product Code:
BODL00173
Size:
96 Assays
Target:
TIMP4
Synonyms:
TIMP Metallopeptidase Inhibitor 4, Tissue Inhibitor of Metalloproteinases 4
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
