The BRSK1 Antibody (CAB17226) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reacts strongly with samples from various species, making it a versatile option for different experimental setups.BRSK1, a member of the AMP-activated protein kinase (AMPK) family, is involved in regulating multiple signaling pathways that impact cell growth and metabolism. By targeting the BRSK1 protein, researchers can gain insights into the mechanisms underlying these pathways and their implications for disease development and progression.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BRSK1 Antibody
SKU:
CAB17226
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SAD-B, hSAD1, BRSK1
Positive Sample:
Raji
Cellular Localization:
Cell Junction, Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Synapse.
Calculated MW:
85kDa
Observed MW:
90kDa
Enables magnesium ion binding activity; protein serine/threonine kinase activity; and tau-protein kinase activity. Involved in mitotic G2 DNA damage checkpoint signaling and protein phosphorylation. Acts upstream of or within G2/M transition of mitotic cell cycle; peptidyl-serine phosphorylation; and response to UV. Located in cell junction; cytoplasm; and nucleoplasm.
Purification Method
Affinity purification
Gene ID
84446
RRID
AB_2768626
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using BRSK1 Rabbit pAb (CAB17226) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Raji cells using BRSK1 Rabbit pAb (CAB17226) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:10s.
Immunohistochemistry analysis of paraffin-embedded Human stomach using BRSK1 Rabbit pAb (CAB17226) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using BRSK1 Rabbit pAb (CAB17226) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
