The BRWD1 Antibody (CAB12656) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the BRWD1 protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for studies in genetics, epigenetics, and cancer research.BRWD1 is a member of the bromodomain and WD repeat domain-containing protein family, and is known to interact with histone proteins to regulate gene expression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
BRWD1 Antibody
SKU:
CAB12656
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
N143, WDR9, WRD9, DCAF19, C21orf107, BRWD1
Positive Sample:
HeLa, Jurkat, SGC-7901, NIH/3T3
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
263kDa
Observed MW:
263kDa
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. This gene is located within the Down syndrome region-2 on chromosome 21. Alternative splicing of this gene generates multiple transcript variants encoding distinct isoforms. In mouse, this gene encodes a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain which may regulate chromatin remodelling and associates with a component of the SWI/SNF chromatin remodelling complex.
Purification Method
Affinity purification
Gene ID
54014
RRID
AB_2759502
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using BRWD1 Rabbit pAb (CAB12656) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
