The C-Reactive Protein (CRP) Antibody (CAB0224) is a high-quality antibody developed for reliable detection and analysis of target proteins. This highly reactive antibody, generated in rabbits, is specifically designed for use in Western blot applications and is validated for its specificity with human samples.CRP is a significant biomarker of inflammation and is commonly used to assess the severity of various conditions, including cardiovascular disease, autoimmune disorders, and infections. The antibody binds to the CRP protein, allowing for the detection and analysis of CRP levels in a variety of samples, making it an invaluable asset for studies in immunology, cardiovascular research, and diagnostics.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
C-Reactive Protein (CRP) Antibody
SKU:
CAB0224
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PTX1, C-Reactive Protein (CRP)
Positive Sample:
Human serum, Mouse liver, Mouse lung, Rat spleen
Cellular Localization:
Secreted.
Calculated MW:
23kDa
Observed MW:
25kDa
The protein encoded by this gene belongs to the pentraxin family which also includes serum amyloid P component protein and pentraxin 3. Pentraxins are involved in complement activation and amplification via communication with complement initiation pattern recognition molecules, but also complement regulation via recruitment of complement regulators. The encoded protein has a calcium dependent ligand binding domain with a distinctive flattened beta-jellyroll structure. It exists in two forms as either a pentamer in circulation or as a nonsoluble monomer in tissues. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection.
Purification Method
Affinity purification
Gene ID
1401
RRID
AB_2757037
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using C-Reactive Protein (CRP) Rabbit pAb (CAB0224) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of L929 cells using C-Reactive Protein (C-Reactive Protein (CRP)) Rabbit pAb (CAB0224) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
