The C14orf166 Antibody (CAB3436) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human biological samples, enabling accurate detection and analysis in various experimental settings.C14orf166, also known as Chromosome 14 Open Reading Frame 166, is a poorly characterized protein with potential implications in several biological processes, including signal transduction and gene regulation. By targeting C14orf166, researchers can gain valuable insights into its role in cell biology, offering new avenues for investigating diseases and therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
C14orf166 Antibody
SKU:
CAB3436
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Cytosol, Microtubule Organizing Center, Perinuclear Region.
Calculated MW:
28kDa
Observed MW:
25kDa/28kDa
Enables RNA binding activity; RNA polymerase II complex binding activity; and identical protein binding activity. Involved in negative regulation of protein kinase activity; positive regulation of transcription by RNA polymerase II; and tRNA splicing, via endonucleolytic cleavage and ligation. Located in microtubule cytoskeleton; nucleoplasm; and perinuclear region of cytoplasm. Part of tRNA-splicing ligase complex.
Purification Method
Affinity purification
Gene ID
51637
RRID
AB_2765111
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using C14orf166 Rabbit pAb (CAB3436) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from NIH/3T3 cells, using C14orf166 Rabbit pAb (CAB3436) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 45s.
