The C5/C5a Antibody (CAB8104) is a high-quality antibody developed for reliable detection and analysis of target proteins. Complement C5 is a protein involved in the complement system, a crucial component of the body's immune defense mechanism.Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By targeting Complement C5, researchers can detect and analyze the protein in various cell types, providing valuable insights into its function in immunology and disease pathology.Complement C5 is known to play a key role in the activation of the complement cascade, leading to the elimination of pathogens and damaged cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
C5/C5a Antibody
SKU:
CAB8104
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C5D, C5a, C5b, ECLZB, CPAMD4, C5/C5a
Positive Sample:
Mouse lung
Cellular Localization:
Secreted.
Calculated MW:
188kDa
Observed MW:
188kDa
This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
727
RRID
AB_2768660
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse lung, using C5/C5a Rabbit pAb (CAB8104) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
