The Anti-Caldeomon Antibody (CAB4884) is a highly specific and sensitive tool for studying the expression and function of caldesmon, a key regulator of smooth muscle contraction. This polyclonal antibody, produced in rabbits, has been validated for use in various research applications, including immunofluorescence, immunohistochemistry, and flow cytometry.Caldesmon is a critical protein involved in the regulation of actin-myosin interactions, essential for the contraction and relaxation of smooth muscle cells. Dysregulation of caldesmon expression or activity has been associated with various diseases, including cardiovascular disorders and smooth muscle tumors.
By targeting caldesmon, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially identify new therapeutic targets.With its high specificity and sensitivity, the Anti-Caldeomon Antibody (CAB4884) is a valuable tool for researchers studying smooth muscle biology, cardiovascular diseases, and cancer. Its ability to detect caldesmon in a variety of sample types makes it an essential reagent for investigations into the role of this protein in health and disease.
Product Name:
Caldesmon Rabbit Monoclonal Antibody
SKU:
CAB4884
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 694-793 of human Caldesmon (Q05682).
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
800
Clone Number:
ARC0315
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Caldesmon Rabbit mAb (CAB4884) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
