The Carbonic Anhydrase 1 (CA2) Monoclonal Antibody (CAB9148) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and shows strong reactivity with human samples, making it an ideal choice for a variety of applications including Western blot analysis.Carbonic anhydrase 2 plays a crucial role in catalyzing the interconversion of carbon dioxide and water to carbonic acid, a process essential for various physiological functions including respiration, kidney function, and acid-base balance. Dysregulation of carbonic anhydrase 2 has been implicated in various diseases including osteoporosis, glaucoma, and certain types of cancer.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Carbonic Anhydrase 1 (CA2) Monoclonal Antibody
SKU:
CAB9148
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1451
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
293T, Mouse spinal cord, Mouse brain, Mouse kidney, Rat spinal cord, Rat brain
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
29kDa
Observed MW:
29kDa
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
760
RRID
AB_2863671
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Carbonic Anhydrase 2 (CA2) Rabbit mAb (CAB9148) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
