The CASP3 Polyclonal Antibody (PAC015934) is specifically designed for research involving CASP3, a key enzyme in the execution phase of cell apoptosis. The antibody, produced in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blot and immunohistochemistry. By targeting the CASP3 protein, this antibody enables precise detection and analysis in different cell types, making it an excellent tool for studies in cell biology and disease mechanisms.
CASP3, also known as caspase-3, is a crucial mediator of programmed cell death and is involved in various cellular processes such as DNA repair, cell differentiation, and immune response regulation. Dysregulation of CASP3 activity is linked to numerous diseases including cancer, neurodegenerative disorders, and autoimmune conditions. Investigating the function of CASP3 is essential for developing targeted therapies that modulate cell survival and death pathways in these pathological states.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO15934(CASC3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 293T cells, A549 cells, Primary antibody: PACO15934(CASC3 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO15934(CASC3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The product of this gene is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. It is predominantly located in the Cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles.
Synonyms:
ancer susceptibility candidate 3
UniProt Protein Function:
CASC3: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA- helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer. Belongs to the CASC3 family.
UniProt Protein Details:
Protein type:RNA-binding; Translation
Chromosomal Location of Human Ortholog: 17q21.1
Cellular Component: perinuclear region of cytoplasm; nuclear speck; cytosol; ribonucleoprotein complex
Molecular Function:identical protein binding; protein binding; enzyme binding; ubiquitin protein ligase binding
The product of this gene is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. It is predominantly located in the cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles. [provided by RefSeq, Jul 2008]
protein CASC3; MLN 51; barentsz; protein barentsz; metastatic lymph node 51; metastatic lymph node gene 51 protein; cancer susceptibility candidate gene 3 protein
UniProt Protein Name:
Protein CASC3
UniProt Synonym Protein Names:
Cancer susceptibility candidate gene 3 protein; Metastatic lymph node gene 51 protein; MLN 51; Protein barentsz; Btz
