The Caspase-10 Antibody (CAB0216) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By targeting the caspase-10 protein, this antibody enables precise detection and analysis in a variety of cell types, offering insights into apoptosis regulation and cell survival mechanisms.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Caspase-10 Antibody
SKU:
CAB0216
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCH4, ALPS2, FLICE2, FLICE-2, Caspase-10
Positive Sample:
Jurkat treated with Etoposide
Cellular Localization:
Cytoplasm, Cytosol, Ripoptosome.
Calculated MW:
59kDa
Observed MW:
17kDa
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
843
RRID
AB_2757030
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using Caspase-10 Rabbit pAb (CAB0216) at 1:1000 dilution. Jurkat cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
